Electrochemiluminesence Epidemiologic Detection of Toxoplasma gondii Infection in Pregnant Women With Direct and Indirect Diagnostic Techniques (ELISA Avidity Plus Biochemical Assay)

Background and aims: Toxoplasmosis is a very common disease in the world. Two types of acquiredtoxoplasmosis have been detected. In the chronic toxoplasmosis, the abnormality of tissue functionis little but, in acute toxoplasmosis, function of RES system becomes interrupted. The assessment oftoxoplasma antibody with tissue enzymes in this stage is very important. Furthermore, serum ferritinin some conditions became high in acute phase of infectious disease.Methods: This study was based on comparative abundance study. Materials consisted of 980 serumand amniotic fluid samples collected from human blood with high level of IgG antibody againstToxoplasma gondii in Rajaie center, Tehran, Iran. The standard test was ELISA assay to detect theseantibodies and the main test was measurement of liver transaminases (SGOT, SGPT) bilirubin andferritin to evaluate acute toxoplasmosis. Data analysis was done by SPSS version 18.0. This study wasdone during March to June, 2017.Results: Results showed that in some patients with high level of IgG AVIDITY antibody against T. gondiithe level of liver transaminases, serum bilirubin and ferritin became increased. For example in 120patients with acute toxoplasmosis the mean serum levels of SGOT were 108 IU/L and in 80 patientsthe mean serum bilirubin were about 5 mg/dL.Conclusion: In acute congenital toxoplasmosis, the evaluation of IgG AVIDITY was first step and thenthe measurement of biochemical factors such as serum transaminases, serum Bilirubin and serumFerritin were important.